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There were 86 (0.5%) highly expressed genes, 2,850 (17%) medium expressed genes and 13,956 genes (82.5%) with low expression in transition lactation MSC (Table 1).
In both the Holstein and Jersey breeds, B4GALT1 and TSTA3 showed the highest expression in transition milk samples.
Only ST8SIA1, which regulates synthesis of GD3, the most abundant ganglioside in early lactation bovine and human milk [38], showed a higher expression in transition milk.
Figure 2 illustrates a link between consistency of differential expression in transition from norm to cancer and expression variability in the norm.
SCD1 showed higher expression in transition lactation and decreased expression in peak and late lactation MSC.
Most of the genes involved in lipid metabolism showed high expression in transition lactation MSC.
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In the third experiment (CL1/trans vs. CL1/exp), 26 genes had a different expression level in transition phase, including 10 genes with increased expression (bioB, bioY and phoD) and 16 genes with decreased expression (e.g. seuC, seuB, ssuD2, ssuC, ssuD1 and ssuB) (Fig. 3B).
Only seven genes in Holstein cows and six genes in Jersey cows had more than two-fold higher RPKM expression values in transition milk (Figure S1).
Two miRNAs exhibited relative increased expression in the transition from normal cervix to atypical dysplasia and decreased expression in the transition from atypical dysplasia to cervical carcinoma, namely miR-522* and miR-512-3p miR-512-3p miR-512-3p
Six miRNAs displayed relative decreased expression in the transition from normal cervix to atypical dysplasia and increased expression in the transition from atypical dysplasia to cervical carcinoma, namely miR-106a, miR-205, miR-197, miR-16, miR-27a and miR-142-5p miR-142-5p miR-142-5p
To elucidate structure function relationships, we explored the three-dimensional crystal structure of an ASP (called Ac-ASP-7), which is highly up-regulated in expression in the transition of A. caninum larvae from a free-living to a parasitic stage.
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