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(D) PCR amplification and real time PCR analysis of c30f12.4 gene expression in total RNAs from the N2 and c30f12.4 mutant.
a, b Western blots showing the changes in HIF-1α expression in total protein lysates isolated from Beas2b cells treated with MG-132 (5 μM) and/or cysteamine (250 μM) for 12 h.
In addition, we determined Fmr expression in total brain homogenates by Western blot analysis.
To assess whether 5 hours of mechanical ventilation influenced the Ang-Tie2 system, we determined Ang-1, Ang-2 and Tie2 expression in total lung homogenates.
As shown in Fig. 1D, the level of intracellular FoxP3 expression in total FoxP3+ cells (based on the mean fluorescence intensity) did not vary after basiliximab injection.
To determine changes in protein expression in total muscle and microsomal fractions, 25 µg of protein was subjected to SDS-PAGE and transferred to nitrocellulose.
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We compared gene expression patterns in total placenta homogenates, for male and female offspring, by the RT-qPCR analysis of 20 imprinted genes.
The KREPA3, 4, 5, and 6 proteins appear particularly essential for editosome integrity since knockdown of KREPA3, 4, or 6 expression results in total loss of editosomes [49] [53].
The signatures of CD4+ and CD8+ T-cell subsets identified a greater differential expression than in total CD3+ cells.
With respect to phase II metabolism, expression of in total 89 enzymes was analyzed (see additional file 2).
RT-PCR was used to detect SNCG mRNA expression levels in total RNA samples extracted from four cell lines.
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