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Important steps in this regulation take place at the level of chromatin, where the (epi genomic environment of a gene determines its expression in time and space.
Here we use a recently developed high throughput methodology for experimental cis-regulatory analysis to elucidate the genomic regulatory system controlling alx1 expression in time and embryonic space.
Multiple mRNAs that encode proteins that are functionally related often interact with RNA-binding proteins (RBPs) and noncoding RNAs (ncRNAs) that coordinate their expression in time and space as RNA regulons within the ribonucleoprotein (RNP) infrastructure we term the ribonome.
To examine whether a BMP signaling pathway functions in specification of cell fates in sea urchin embryos, we have cloned sea urchin BMP2/4, analyzed its expression in time and space in developing embryos and assayed the developmental consequences of changing its concentration through mRNA injection experiments.
In animals, miRNAs are involved in processes such as tissue development and cell differentiation [3], apoptosis [4], in which fine regulation of gene expression in time and space is required for the correct execution of these processes; and in diseases such as cancer [5].
EP enhanced SHP-1 protein expression in time- and dose-dependent manners.
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A transgenic mouse C57BL/6 strain expressing E (enhanced) GFP under the control of the Cldn5 promoter, denoted Cldn5 BAC -GFP, allowed direCldn5 BAC -GFPf Cldn5 promoter allowedy andirecteby, Cldetectionssiof in time-lapse imaging.
DOI: http://dx.doi.org/10.7554/eLife.03743.008 > -wrap-foot>> -wrap-foot>> -wrap-foot> To determine the temporal relationship between Pitx2 and cVg1 in whole embryos and during embryonic regulation, we compared their expression in time-course.
Moreover, we investigated the effects of Xag inoculation on gene expression in time-course experiments with susceptible and resistant NILs, which revealed a significant increase in the expression of NBS-LRR genes located on QTL previously associated with BLP resistance.
However, some genes did show differential expression levels in time between the treatments (chemotaxis-inducing root exudate, a non-inducing root exudate, 0.1% sucrose and water) (Table 2).
To determine the temporal expression patterns of the genes responsible for the rate change phenomenon during differentiation, we classified the 3505 genes into categories according to gene expression patterns in time.
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