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Our experimental data showed that ECM gene expression was higher in the infarct region than the expression in the remote region, and ECM gene levels in the remote region were higher than that in the LV of controls, indicating a local source of ECM production.
The key factors were pre-targeted by examining the most significant changes in ECM gene expression in the infarct region at day 7 post-MI, compared to gene expression in the remote non-infarcted region of the same LV and in the LV from control group.
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In contrast, the magnitude of [18F]FEDL binding pancreatic tissue was significantly lower in the regions remote from the site of tumor growth (red rectangle Fig. 4B, shown magnified in 4D), which was consistent with low (background levels of HIP/PAP expression in these remote regions of pancreas (Fig. 4G).
(C) Collagen type I and III mRNA expression in the adjacent and remote myocardium of SHAM and MI.
Expression in remote myocardium is set at one, and expression in infarcted tissue is calculated as a proportion of the expression in corresponding remote myocardium.
The sprouting vessels in the myocardial infarct showed upregulation and expression of β3 integrin, whereas only a faint expression in the muscle cells in the remote myocardium was observed (Figure 2).
Interestingly, the Conjugates could effectively improve phosphor-Stat3 expression in both remote and border zones, to investigate whether Stat-3 was essential in the Conjugates' anti-fibrosis effect, Stat3 inhibitor and siRNA were used.
Quantitative RT-PCR analysis of the genes encoding the Ca2+-independent transient outward potassium current (Ito) revealed that the transcript expression of Kv1.4 was up-regulated in the remote left ventricle only in untreated rats.
There were no differences in the inflammatory response in the infarct/border area 24 h or 4 weeks after the MI, but in the remote myocardium expression of CD68 (macrophages) was significantly higher in TG rats 4 weeks after the MI (2.8 fold upregulation, P = 0.0003).
The muscle as reference tissue because it has now HIP/PAP expression, while the possibility of HIP/PAP expression in remote regions of "normal" pancreas could not be excluded a priori.
However, CD45-positive leukocytes could not be detected by immunostaining in the remote area of the heart where MIRT1 expression is observed.
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