In correspondence with SIP1, SNAI2, and TWIST2 expressions in the pit region, FN1, SMTN, LMOD, MEF2C, PDGFRB, EDNRA, SLIT2, ROBO1, WNT5A, and ROR2, preferentially expressed in the pit region, whereas CDH2 expressed in the gland region concordant with TWIST1 expression.
Embryos with GFP expression in the olfactory pit region were selected for further analyses and stained with an HuC/D antibody.
Embryos with GFP expression in the olfactory pit region were selected for further analyses, and differentiated neurons were detected by HuC/D antibody staining.
CagA expressing strains of H. pylori have also been shown to increase mcl-1 expression in the gastric pits of infected Mongolian gerbils (25).
The expression of Shh in the pit may differ between FVB mice utilised in this study and mouse strains utilised in other studies (van den Brink et al, 2001; Fukaya et al, 2006).
These data suggest that mesenchymal-like gene expression of the pit cell in both mice and humans is induced by Hh signal activation.
We have, however, confirmed Shh RNA expression by LCM, in which high expression of Shh was detected in the pit region of FVB mice.
In transgenic mouse models of hypergastrinaemia, gastrin induces CCK-2 receptor expression in gastric pit cell precursors (Nakajima et al, 2002) and invasive gastric carcinoma (Wang and Dockray, 1999).
Finally, we detected Shh expression in pit and parietal cells of the gastric mucosa, whereas a recent study has detected Shh only in parietal cells (Fukaya et al, 2006).
Moreover, as in the pituitary gland, Pit-1 regulates GH expression in the MCF-7 human breast adenocarcinoma cell line (Gil-Puig et al. 2005).
