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Using a transgenic reporter strain, we found that 7 days of iCH in mature adults induced gata4 EGFP expression in the outermost layer of cortical muscle (9/10 iCH, 1/11 controls; Figure 8G,H).
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An Arabidopsis counterpart gene has been found to be exclusively expressed in the outermost (L1) layer of the SAM [ 28].
While no GFP activity could be detected in uninjured hearts, strong GFP expression was evident in the outermost cell layers of the heart at 3 days post amputation (dpa) and still detected at 7 dpa (white arrows in Fig. 5B).
We find that the ovule specific expression produced by the INO promoter in the outermost cell layer on the gynobasal side of the outer integument is a result of a combination of position-specific and general enhancers to promote ovule expression, and repressive sequences to prevent expression outside of the ovule.
It is expressed in two bilateral regions in the outermost cell layer in the animal hemisphere of the embryo.
Serial sections indicated that expression was highest in the anlagen and primordia in the outermost 2 to 3 cell layers of flower primordia.
Expression of CK5 in these lesions was most intense in the outermost, basal-like layer of cells, and in all lesions multiple layers of CK5-immunoreactive cells were seen (arrow, Figure 2b).
Overexpression of PKC η in human keratinocytes partially inhibits DNA synthesis and induces epidermal transglutaminase, which is involved in the crosslinking of proteins in the outermost epidermal layers, but does not change the expression of K1 and loricrin.
The cytokinin mainly accumulated in the outermost layers of the ARP, where an almost total absence of PIN1 expression and auxin accumulation were observed.
Interestingly, the triose phosphate transporter located in the outermost membrane of the apicoplast, PFE0410w, was under-expressed under artesunate.
Initially ClpostHox expression is detected in a restricted area of about 30 cells in the posterior region of the embryo, in the outermost cell layer.
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