Since the 15 miRNAs identified, which include the 4 miRNAs used to normalize our data (see Methods), showed rather consistent expression levels in the extensive list of tissues we surveyed, they would be strong candidates for normalizing miRNA expression should the types of normal human tissues beyond our list be examined.
Vertebrate Gsx genes (usually called Gsh1 and Gsh2) have anterior boundaries of expression in the brain with extensive expression posteriorly into the neural tube, in a dorso-ventrally restricted fashion that may be comparable to Drosophila [ 11- 17].
While not comprehensive of all the genes observed with differential expression in the microarray analysis, the extensive qPCR confirmation experiments validate the quantitative accuracy of the discovery experiments.
This positive correlation with gene expression was also detected in the extensive overlap between Cbt occupancy and trimethylated histone 3 lysine 4 (H3K4me3).
In addition, the crystallin genes probably had a different function, outside of the lens, before being recruited to lens function, as evidenced by a more distantly related gene, with expression in the liver (a more extensive phylogenetic analysis of the gene family could be used to more formally establish ancestral function).
For many years the pleasing speaking voice of Julian Budden, who has died aged 82, with its easy, friendly manner, was to be heard in the intervals of BBC radio broadcasts of Verdi operas, unravelling the context and the genesis of each work with the calm authority that found more permanent expression in his extensive writings.
Our results show that germline-dependent expression in the non-gonadal soma is extensive.
Immunocytochemistry of larval nervous tissue overexpressing TBPH using the D42-GAL4 driver showed extensive expression in the cytoplasm of motor neurons.
We found the absence of RIP1 and RIP3 expression in normal hepatocytes but extensive expression in the liver from APAP- or ConA-treated mice on immunohistochemical analysis and western blotting.
ASCL1 and DLX2 maintained high expression in the proliferative zones and showed extensive immunofluorescent double-labeling with the cell division marker Ki-67.
