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Exposure to IL-1 and TNFα in vitro significantly upregulated TRPV1 expression in challenged cartilage (average relative expression 3.34 (95% CI 2.55 to 4.37) versus 1 (95% CI 0.68 to 1.48) (n=4) (figure 2A).
At both tissue harvest times, more genes increased expression in challenged groups compared to NV-NC control groups 55-788%) and more increased in severe compared to mild pathology group on day 5 (66%).
To investigate the regulation of miRNAs in MAC-T cells in response to stimulation by pathogens, miRNA expression in challenged cells at different time points were compared to expression in unchallenged cells (controls) at the same time point using DEseq and raw reads as suggested [ 29].
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In contrast, expression was obvious in challenged eggs.
Milk TGF-β1 did not significantly differ in response between any of the challenge groups; however, a trend towards altered TGF-β1 expression in animals challenged with Newbould 305 was observed, whereby the cytokine levels reached a maximum at 48 hours pi (approximately 4000 pg/ml, data not shown).
A recent study however, reported hepatic MCP-1 protein expression in mice challenged with LPS [ 28].
In our study, quantitative PCR revealed an increase of ferritin expression in clams challenged by QPX after 28 and 48 days.
Our results indicated the enhanced 3-NT expression in HUVECs challenged with homocysteine, which was attenuated by pretreatment with α-ZAL.
Western blot indicated the enhanced 3-NT expression in HUVECs challenged with homocysteine, which was attenuated by pretreatment with α-ZAL.
Gene expression in CHDR challenged with LPS PMA exhibited a circa 5-fold upregulation on days 3 and 7, LPS PMA, day 7, P < 0.05, 24.8 ± 19.9; control 5.5 ± 4.6).
105 Characterization of the changes in protein expression in calves challenged with M. haemolytica by nano-liquid chromatography-tandem mass spectrometry (LC-MS/MS) 42 identified ITIH4, an acute phase protein commonly associated with chronic obstructive pulmonary disease in humans, to be elevated, along with haptoglobin, a known acute phase marker for BRD.
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