Specifically, type I IFN acts to increase MHC class I expression for antigen presentation [ 31], promote T cell survival [ 32], inhibit cell proliferation [ 33], mediate apoptosis [ 26], and increase NK cell activity [ 34].
Type II IFN acts to increase both MHC class I and II expression for antigen presentation [ 29], stimulate macrophages to kill engulfed pathogens [ 35], induce apoptosis [ 36], and regulate leukocyte-endothelium interactions [ 37] in addition to many other immune-related activities.
Mean, SE, and statistics were performed on the ΔCT data and only converted to relative expression levels (2̂ΔCT) for presentation in the figures.
Mean, standard error and statistics were performed on the ΔCT data and only converted to relative expression levels (2∧ΔCT) for presentation in the figures.
Mean, standard error, and statistics were performed on the ΔCT data and only converted to relative expression levels (2ˆΔCT) for presentation in the figures.
Thus, for simplicity and ease of presentation, we have averaged the gene expression data across cultures for presentation in the figures.
Mean, SEM, and statistics were obtained for the Δ C T data and converted to relative expression levels (2Δ C T) for presentation.
This allows the rationale design of improved Salmonella carrier strains and the development of novel strategies for the expression and presentation of recombinant antigens.
A detailed presentation of the expression for specific MHC genes can be found in a separate supplementary text (Additional files 2 and 3).
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