While it may seem confusing that we control co-expression for expression level, note that co-expression is defined as correlated up- and down-regulation across measurements in time-course experiments.
Phosphorylated 4EBP1 expression was assessed for both cytoplasmic and nuclear expression, nuclear expression for pAKT1 and cytoplasmic expression for pS6.
The plasmid loading regulated the quantity and duration of transgene expression, with expression for 105 days achieved at the highest dosage.
B: KHC-2 cells with reconstituted USP18 expression maintained expression for the duration of growth in mice.
While calculating fold change for gene expression changes, expression values for A. glycines fed with susceptible soybean SD01-76RR) were taken as reference.
For baculovirus expression, pFastBac expression vectors (Bacvector 3000, Novagen) were used.
For protein expression: +, expression; ++, overexpression; -, no expression; ND, not determined.
Cells can always be re-sorted for eGFP expression if expression levels drop significantly.
Stable transfections were achieved with a tetracycline-controlled gene expression system for expression of Src.
The amplified tF1 has been cloned in bacterial expression vector, pET21b for expression in Escherichia coli.
random: a random real number between 0 and 1. int [expression]: the lowest integer for expression.
