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For infection with the GFP-expressing viruses for miRNA expression, flow cytometry analyses (FacsCalibur, Becton Dickinson) were performed to confirm that 90% of cells were infected.
In addition, 1b up-regulated p21CIP1/WAF1/Sdi1 protein expression (flow cytometry), a cell cycle inhibitor, reduced cell growth, and induced DNA fragmentation (increased sub-G1 phase cells) and phosphatidylserine externalization in plasmatic membrane (increased annexin V positive cells).
To check whether Her2-S-Fab can bind to of cells with Her2 expression, flow cytometry analysis was performed using both HER2 positive and HER2-negative cells.
Might, for example, more appreciation for freedom of expression flow Eastward and a greater appreciation of governing in the common interest and long-term perspective flow Westward?
After induction of protein expression, flow cytometric data was collected on a FACSCalibur System (Becton Dickinson) at 0 and 8 hrs post induction.
Osteoclast differentiation was evaluated by gene expression, flow cytometry, tartrate-resistant acid phosphatase staining, fluorescence and electron microscopy.
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Spin, footwork, covering the ice, speed, height expression and flow.
Proliferation was then measured by detection of Ki67 expression using flow cytometry.
We measured GFP expression via flow cytometry with and without induction with β-estradiol.
We next infected these cells with WT or G28A Jc1 and monitored NS5A expression by flow cytometry.
After then, T cells were measured by detection of CD69 expression using flow cytometry or used for RNA extration.
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