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Unless, both genes are expressed at a similar level, this procedure will cause wrong expression estimates and should thus avoided.
Validation of RNAseq-based expression estimates for selected genes was attempted by qPCR.
Validation of RNAseq-based expression estimates for selected genes, of particular biological interest, was attempted by qPCR.
Technical noise may be modeled with a log-normal function to adjust gene expression estimates.
Another perl script was used to isolate the mRNA expression estimates (*.isoforms.normalized_results files) for the next steps.
If spike-ins are available, they can be used to estimate individual cell's RNA content and normalize expression estimates more accurately.
The GCRMA algorithm was used to obtain expression estimates [79].
Mean posterior expression estimates for each gene were log2 transformed.
An interaction effect would be observed if expression estimates between strain groups clade8stx2 and clade2stx2 are different from expression estimates between strain groups clade8stx2,2c and clade2stx1,2.
Afterwards, quantile scaling and WPP algorithm were used to normalize and calculate expression estimates [29].
Our results demonstrate that probe selection improves gene expression estimates from exon arrays.
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