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Focusing on an N-terminally truncated (Δ108) variant of ST6Gal-I previously shown to represent a minimally sized, still active form of ST6Gal-I, we show here that protein expression engineering and optimization of bioreactor cultivation of P. pastoris KM71H (pPICZαB) synergized to enhance the maximum enzyme titer about 57-fold to 17 units/L.
1) Because detection of gene expression, engineering cell model is unnecessary.
Productivities obtained in aqueous batch reductions were 21-fold improved by addition of 20% (v/v) hexane, NAD+, expression engineering, cell permeabilization and pH optimization.
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While many of these bioprocess and culture media improvements were kept as trade secrets [ 7], cell line development technologies like expression vector engineering, cell line engineering and clone screening technologies were extensively reviewed [ 1, 7- 10].
Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity.
Despite significant advances of CRISPR technology development to editing the genome sequence or regulating gene expression, genome engineering, broadly defined, is still in its infancy for studying and engineering diverse features inherent to the genome.
However, knowledge of an enzyme's amino acid sequence is essential for its recombinant expression and engineering.
Although Fuller had no academic credentials, his ideas found expression in engineering, architecture and what he called "energetic-synergetic geometry," or geometry as found in nature.
This contribution will spur future studies in the heterologous expression and engineering of lantibiotics.
And I didn't even open the dihedral doors, which arc out and up in a brazen expression of engineering chutzpah.
She described the bridge as "a beautiful expression of engineering design", and noted that the prestressed concrete stays were "a common feature of bridges designed by Morandi in the sixties".
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