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Moreover, a quorum sensing regulatory gene encoding the quorum threshold expression element QteE [ 38] also was differentially expressed in parS and parR mutants (Table 1).
To obtain the optimal expression element, successive investigation of RBS sequences and promoters was conducted.
Instead of pursuing the same failing transaction-oriented business model, imeem is focused instead on maximizing ad impressions by emphasizing music discovery and providing users with what Jang calls "an expression element" with customized personal pages and the creation and sharing of song and video playlists.
The Creative Expression element of No Name-Calling Week has been particularly popular at our school, giving students the chance to funnel their feelings about bullying and name-calling into poems, graphic novels, paintings, sculptures, dioramas, murals, short films and songs.
Equal molar expression of TCR α and TCR β-chain genes was made possible by the inclusion of a T2A expression element between the TCR α and β chain genes [35].
For instance, in one of the most studied RNA thermometer called the ROSE (Repression Of heat-Shock gene Expression) element, a guanine residue at position 83, paired opposite the Shine-Dalgarno (SD) sequence in a hairpin structure is known to play a prominent role in the ability of the mRNA to change its expression with temperature [4].
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Second, pOmni is a pro/eukaryotic dual-expression vector containing compatible eukaryotic CMV expression elements and prokaryotic T7 expression elements, which would greatly benefit researches in which both prokaryotic and eukaryotic expression of a target gene are necessary.
To construct an efficient vector system (Fig. 1a), we analyzed the efficiency of different expression elements in rice.
Here, we show the rapid construction of expression vectors containing three or four tandemly arrayed cDNA expression elements and their expression in mammalian cells.
Whereas the standard destination vector contains only CmR and ccdB genes flanked by two attR signals, this destination vector contains, in addition, one or two cDNA expression elements.
Furthermore, the target gene cloned in pOmni is ready to be high-efficiently expressed in either Escherichia coli cells or eukaryotic cells because of the elaborate design of compatible T7 promoter and CMV promoter expression elements in the vector.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com