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P. pastoris demonstrated ample potential for high-level expression, efficient secretion and growth rate with very high cell densities.
We found no role for Gc in recruitment of N, genome and RdRp, however Gc is necessary for optimal Gn expression, efficient production of RVF-VLPs and possibly, infectivity.
Despite the positive benefits of the new system we have described- increased expression, efficient folding and high purity- the method does have potential limitations.
We have shown that, by using a stepwise strategy to address specific problems relating to codon optimisation, periplasmic protein translocation and tight regulation of expression, efficient bacterial expression of DI of human β2GPI is possible.
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Despite integration in regions rich in epigenetic marks associated with gene silencing, lentiviral vector expression remained efficient.
In order to develop a general expression for efficient metric computation, consider the binary sparsity vector obtained at the (p−1 -th iteration: ({mathbf {d}}^{(p−1 -thast }).
This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.
In order to evaluate the multi-parametric harvesting performance, an expression of efficient harvesting entropy was proposed based on the entropy generation distributions of thermal harvesting schemes.
We show that psiUx ensures high levels of expression and efficient knock down of the target gene also in stable cell lines.
Using lepidopteran insect cells as an expression platform, efficient expression of mosquito ORs was achieved for the first time.
For rapid testing of protein expression and efficient purification, a His6-tag was fused to the C-terminus of the hCRs in the attB-PCR step.
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