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Expression efficacy was tested using eye-specific GMR-Gal4 activation.
The expression efficacy of rl reporter gene in tumors established from p3G-transfected cells was demonstrated by bioluminescence imaging).
A careful balance between plasmid DNA loading, subsequent transgene expression efficacy, and cytotoxicity therefore needs to be maintained for optimizing performance using polymer nanoparticle nanoassemblies.
42, 48, 49 A correlation between the number of 28-bp repeats and gene and protein expression efficacy has been repeatedly found and confirmed by our investigations.
The synthetic gene was investigated for its expression efficacy and ability to sensitize mammalian cells to CB1954 using western blotting analysis and cytotoxicity assays.
Many of these quantitative differences in gene expression efficacy are consistent with the potent agonist activity of EE and the weak agonist activity of TAM.
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The consistency of results obtained from different research groups and identification of improved gene expression efficacies of RAFT based gene delivery vectors indicate the scope of this polymerization approach for future studies.
However, as demonstrated here and previously by us, several poly amino ethers) demonstrate either higher transgene expression efficacies, nanoparticle templating activities, or both, compared to pEI25k.
Several candidates among the new PAEs synthesized in our laboratory demonstrated higher transgene expression efficacies compared to 25 kDa poly ethylene imine), or pEI25k.
Plasmid DNA-loaded 1,4C 1,4Bis GNPs or "nanoassemblies" were delivered to 22Rv1 human prostate cancer and MB49 murine bladder cancer cells in order to evaluate their transgene (luciferase) expression efficacies.
In particular, changes in expression and efficacy of glutamate transporters have been reported.
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