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Detection success largely depends on two parameters: signal strength (the fraction of GFP-positive cells in the animal and how brightly each cell fluoresces) and expression duration (a transiently expressed gene is less likely to be detected).
The different technologies are functionally graded with respect to safety, expression magnitude and expression duration.
In particular, the cationic polyrotaxanes displayed sustained gene delivery capability in HEK293 cells in both serum and serum free condition with the increasing expression duration.
Although the data here did not examine longevity, other studies our laboratory using different plasmid elements has resulted in an expression duration of several months [43].
We transfected MC.GFP and MC.hAR in HEK293T cells to measure expression duration.
Minicircle DNA, which is devoid of bacterial unmethylated CpG sequences, reduces immune response caused by unmethylated CpG, enhances expression level, and prolongs expression duration [ 16].
Similar(52)
However, we did not detect impairment in estrus expression, estrus duration or ovulation (P > 0.05).
This creates potential to regulate both the level of expression and duration of expression of a cloned gene in differentiated muscle.
The low current density of ChD4 was improved only by prolonging the expression time duration.
12 CAG repeat size modulates clinical expression, disease duration and phenotype.
However, there was no association between NPTX2 expression and duration of the disease.
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