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The pairs of CF4ab vs control and CF4ac vs control shared the most commonly differentially expressed genes, up to 1793 8366 up-regulated and 952 down-regulated with consistent expression direction, and five with opposite expression direction), suggesting the F4ab and F4ac ETEC infections caused more similar response in the IPEC-J2 cells.
(B) Highlight on the 52 genes that have expression direction consistent with decreases in activation of mononuclear leukocytes process.
For example, 52 genes included in the function 'activation of mononuclear leukocytes' showed expression direction consistent with the decrease of the function, therefore suggesting an anergy of those cells.
We developed custom Perl scripts to create a list of genes that was associated with each identification tag and differential expression direction.
To compare differentially expressed genes of inoculated vs. non-inoculated between A line and B line, there were 67 genes shared (28 up-regulated and 36 down-regulated genes with consistent expression direction and three genes with opposite expression direction) (Fig. 2B, Table S5).
We then identified prognostic categories that were reported in greater than 3 independent studies and stratified these by number of genes, differential expression direction, and number of independent studies (Table 3).
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Surprisingly, only 9.6% of these genes were replicated with concordant expression directions in more than one study.
We developed a customized Perl script that incorporates the comparison conditions and identification tags in an algorithm to summarize the expression directions of each mapped gene.
In poor prognosis (includes decreased overall survival, disease free survival, or response to therapy) AML, our comparison showed increased expression of several HOX/TALE genes, specifically HOXA4, HOXA10, HOXB5 and PBX1, while showing decreased expression of MEIS1 and contradictory expression directions of HOXB2 and PBX3.
Additionally, single probe sets and values assigned to each single transcript are displayed in a separate column to identify ambiguity and divergent expression directions of particular probe sets.
Importantly, these 30 DE miRNAs targeted 1,198 mRNA genes, among which 48 were DE and reversed their expression directions going from NC to CA and to CCA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com