The paper discusses assets and drawbacks of stability engineering using the correlation between expression density on the yeast surface and thermal stability with respect to the quality control system in yeast.
PSMA is a type II integral cell-surface membrane protein that is not secreted, and its expression density and enzymatic activity are increased progressively in prostate cancer compared to normal prostate epithelium, thereby making PSMA an ideal target for monoclonal antibody imaging and therapy.
Total receptor expression density varied more from family to family, with Glutamate and Adhesion family receptors exhibiting the highest expression density for Nt_BRIL constructs (Fig. 4B).
In general, Nt_BRIL constructs had higher expression density than ICL3_BRIL constructs for Rhodopsin, Frizzled/Taste2, and Adhesion family receptors, while ICL3_BRIL constructs performed better for Glutamate family receptors.
Density was calculated using the following expression: Density kg / m 3 = Mass kg / Volume m 3. Open image in new window.
Isl2, LIM-homeodomain transcription factor, is expressed exclusively and strongly in contralaterally projecting RGCs throughout the retina with a decrease of expression density in the ventral-temporal crescent where all ipsilateral projections originate (Pak et al., 2004).
Our own data have already hinted towards a strong dependence of splenic tracer uptake on the respective mouse strain used [31], whereas tracer uptake in human LNCaP xenografts accurately reflects the respective expression density of human PSMA and thus allows valid comparisons [13, 25, 30, 32].
In contrast, ionizing irradiation had no effect on the expression density of HLA-E on immortalized microvascular HMECs (Figure 6C).
Based on their CD56 receptor expression density human NK cells can be distinguished as CD56dim or CD56bright NK cells.
The incomplete correlation may result from previously discussed limitations of the DNA-content assay and/or from Ki-67's variable expression density during the cell cycle.
The content of SSEA1 positive cells was analyzed by FACS and revealed 16% positive cells with a low expression density on most of the cells (Fig. 7c).
