This chapter discusses different human-compatible heterologous gene regulation systems, their adaptation to specific expression configurations, and their potential to be integrated into higher order control systems to achieve next-generation gene therapy and tissue engineering strategies.
If the cell-type specific genomic expression configurations are attractors, then they are "pre-programmed" by the particular wiring diagram (architecture) of the GRNs.
However, these 152 experiments are absolutely relevant because they represent the observable and biologically attainable gene expression configurations of the organism.
How are the steady-state gene expression configurations that characterize each cell-type attained?
This approach enabled us to compare simulated gene expression configurations when using GRNs that differed from those reported before.
Our simulations suggest that model B', which assumes that CLEX is a negative regulator of WOX5 and that SCR expression is independent of JKD activity, renders gene expression configurations reproducing the available experimental data.
Indeed, soon after cells depart from the QC, they attain distinct gene expression configurations, each characterizing a set of SC or initial cell types that eventually give rise to the distinct cell lineages conforming the mature root.
This result suggests that some key components have been uncovered and that these components, given some additional newly predicted interactions, are sufficient to recover gene expression configurations that resemble those known for the main cell types within the root SCN.
We found that to recover the observed gene expression configurations in the right cell and spatial location, it was necessary to set SCR to 1 in the initial condition.
We have pioneered binary ON- and OFF-type systems as well as compact bidirectional, auto-regulated and self-regulated one vector expression configurations for regulation of intracellular as well as secreted proteins in mammalian cells.
