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Utilizing the optimized expression conditions, we expressed and analyzed both libraries by SDS PAGE gel electrophoresis and Western blotting.
The GFP RTX protein was expressed poorly under the expression conditions tested.
Tissue-specific genes could be co-expressed in other tissues when expression conditions change.
Depending on the expression conditions, it was possible to express either native or tagged proteins from the same expression vector set.
All mutant inhibitor proteins were expressed in E. coli (BL21DE3) using optimized expression conditions and purified by His-tag based affinity and size exclusion column chromatography.
These expression conditions differ from previously-published resilin expression methods and are recommended when expressing proteins with a larger number of repetitive resilin sequences.
Small-scale expression conditions were tested for all these constructs.
The expression conditions and scale-up production were also studied.
For comparing enzyme activities after applying these different expression conditions, whole cell biotransformations were conducted.
Wild-type Δku70 was subjected to the expression conditions and analysed as a control.
A response surface method was adopted to optimize the expression conditions of the recombinant protein.
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