Sentence examples for expression condition from inspiring English sources
Gag proteins were expressed under the optimal expression condition and purified via gel filtration chromatography.
The ETK-FL protein with His6-tag in the N-terminus was expressed in E. coli membrane through expression condition optimizations.
As previously discussed, it was necessary to evaluate the soluble fraction of pneumolysin, which is the active fraction, expressed in all 24 different operational conditions, to define the most appropriate expression condition and then compare it with the condition for the best cell growth to determine whether the soluble rPly expression was correlated to cell growth.
According to the diameter of inhibition zone, one transformant of each peptide was selected to analyze the expression condition by Tricine-SDS PAGE.
The optimized expression condition for the recombinant AiiA protein was at 25 °C for 6 h with 1 mM IPTG induction.
The novel recombinant Anti-CD22 scFv apoptin protein was expressed in E.coli BL 21 DE3) using the pET expression vector driven by the strong T7 promoter under optimum expression condition.
Utilizing the optimized expression conditions, we expressed and analyzed both libraries by SDS PAGE gel electrophoresis and Western blotting.
The GFP RTX protein was expressed poorly under the expression conditions tested.
Tissue-specific genes could be co-expressed in other tissues when expression conditions change.
Depending on the expression conditions, it was possible to express either native or tagged proteins from the same expression vector set.
These expression conditions differ from previously-published resilin expression methods and are recommended when expressing proteins with a larger number of repetitive resilin sequences.
