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Around anthesis, when petals completely cover reproductive structures, IaH4 expression concentrates predominantly at the distal petal tips and only few IaH4 expressing cells are detectable in the proximal claw region.
This expression concentrates to the tips of cotyledon primordia in embryo heart stage (Fig. 4E,J).
Thus, the members of this miRNA cluster are expressed simultaneously, and studies in the holometabolan species, like D. melanogaster and B. mori have shown that expression concentrates in pre-metamorphic stages.
We further tested organ specific genes as for instance AINTEGUMENTA (ANT) whose expression concentrates in the central tissue of cotyledon primordia extending into the hypocotyl and excludes the region of the SAM (Elliott et al., 1996; Treml et al., 2005).
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The analysis described above for G1/S phase genes was also done for genes that had previously been shown to have their expression concentrated in the G2 phase of the cell cycle [116], [117], [118], [119], [120].
By immunohistochemistry, we observed a pronounced and patterned increase in proTRH immunostaining in the striatum, with the strongest proTRH expression concentrated in the lateral and ventrolateral striatum including the sensorimotor striatum.
TRPC5 mRNA in situ hybridization indicates high expression concentrated in the deep layers 5/6 and superficial layers 2/3 of the PFC, vSUB, piriform, orbitofrontal, and entorhinal cortices (Figure 4).
The cerebellum showed a gradient of high expression concentrated towards lobule VI (Fig. 4E), similar, but not identical to that seen in garden warblers that performed flights (Fig. 4B), and clearly increased relative to finches that sat still (Fig. 4D and 5B).
In addition, IHC detected remarkably higher levels of vWF protein expression concentrated in the tumor stroma region.
This was opposite to the expression of CBX1A that was widespread in young animals and down-regulated in old animals with expression concentrated in the NSC niche.
In control animals, as expected, ligand binding was observed in previously documented areas, with expression concentrated in the lateral septum, ventral pallidum, bed nucleus of the stria terminalis and anterior hypothalamus (Dubois-Dauphin et al., 1996; Johnson et al., 1993; Tribollet et al., 1991).
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