Sentence examples for expression changes obtained by from inspiring English sources

In order to validate gene expression changes obtained by DNA microarray, qRT-PCR was performed on a RotorGene 3000 instrument (Corbett Research, Sydney, Australia) with gene-specific primers and SybrGreen protocol to monitor gene expression as described earlier [ 23].

The gene expression changes obtained by microarray were positively validated in a head-to-head comparison by RT-PCR for the genes HMG-CoA reductase, lipoprotein lipase, apolipoprotein A1, serum Amyloid A1, fibrinogen, ATP transporter A1 and ATP transporter G1 in a previous study [ 27].

While some explanations can be taken into account, we would favour the following: the aperture of global gene expression changes obtained by computational biology is condensing pathway activities and supports the idea of parallel or equivalent functioning oncogenic activities in individual DLBCLs.

Real-time PCR results were in general consistent with the direction of relative expression changes obtained by RNA-Seq, with a Pearson coefficient R of log2 (Fold Change) equal to 0.53, indicating a positive correlation between qPCR and RNA-Seq data.

Correlation of fold changes obtained by the two gene expression profiling techniques was high (R2 = 0.9653), thus indicating that the microarray data obtained were valid.

Mean values of fold changes induced by 5-AZA-CdR in the constitutive levels of gene expression, measured by quantitative RT PCR assays, were compared with the mean fold changes obtained by microarray analysis.

Table 2 shows the relative quantification of gene expression obtained by RT-qPCR, and the gene expression (fold-change) obtained by microarray for 17 genes.

The addition of both drugs, by enlarge, gave the same repertoire of gene changes as lovastatin alone, although some additional gene expression changes were obtained by the combined treatments.

Following ANOVA analysis, which revealed only inter-group specific differences, a list of group specific gene expression changes was obtained by comparison between defined patient subgroups and controls in R statistical environment as described in Methods.

The fold change in gene expression (L5M vs OHB) obtained by using microarray experiments compared to the fold change obtained by using qRT-PCR gives a correlation coefficient (R) of 0.84.

Fold change obtained by qRT-PCR correlated well with the direction of fold change obtained from the normalized intensity data, confirming the validity of the microarray gene expression patterns (Table S6).