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To analyze the expression change in more detail, we classified the differentially expressed (DE) genes based on their change trend from E9.5 to E13.5.
Furthermore, we illustrated the 3D views of PTGDS protein spot, developed with the PDQuest software, in order to provide a clearer vision of its expression change in the examined groups.
Few of the genes differed in the direction of expression change in different mutant alleles.
These findings raised the question: how does gene expression change in the two strains during coculture?
The majority of the signatures that showed a significant expression change in the xenic culture were down-regulated.
Such studies have tremendous potential for characterising in broad terms how patterns of gene expression change in response to challenge.
We evaluated any given set of parameters by calculating the average expression change in the top 30 ranked genes.
Of interest, the gene with the greatest brain expression change in our prior studies [8], [9] by isolation rearing (Arc) was not affected by Nestlet treatment.
To investigate this statistically, we selected grouping of equal number of random ORFs and assessed their average expression change in wildtype against all genotypes.
However, the genetic variation between clinical samples complicates the situation because allelic variation of gene expression could interfere with expression change in the pathophysiological process.
To define gene sets responsive to a specific stress, we identified genes that show a significant expression change in each condition.
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