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As a control, GFP was expressed alone using the same promoter and expression cassette of a single-copy gene.
The expression cassette was packaged into recombinant AAV9 as previously described76.
De Palma, M. & Naldini, L. Transduction of a gene expression cassette using advanced generation lentiviral vectors.
This generates a MC with the expression cassette, and a miniplasmid (MP) with the replication segment.
Additionally, combination of the individual atn and atl expression cassette was also cloned onto pAUR123.
Fourteen transformants stably carried the KJhyPBase expression cassette, and TR_cKJUMPS was excised from seven of these.
Two positions in the CELO genome were chosen to insert the VP2 expression cassette.
All vectors harbor a synthetically optimized XVE expression cassette, allowing β-estradiol mediated protein expression.
These data will facilitate optimal expression cassette design for salivary gland gene transfer.
Moreover, we demonstrate that these MSTN TALENs can facilitate targeted integration of a mCherry expression cassette or a larger muscular dystrophy gene (dysferlin) expression cassette into the MSTN locus in mouse or human cells.
The correct integration of the late ASFV p72 promoter regulated GFP expression cassette was confirmed by direct sequencing of PCR amplicons spanning the mutagenized locus.
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