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Expression of NFAT mRNA was determined using a customised gene expression card (Applied Biosystems) and real-time qPCR was carried out using the ABI Prism 7900HT sequence detection system.
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During the shoots, Mr. McGinley relied on a mini-trampoline, party music, facial-expression cue cards and a "hype girl" named Brandee to encourage the requisite degree of enthusiasm.
But he was baseball royalty, and his expression on this card says he knew it.
Despite the big hair, she is no run-of-the-mill Bond girl; with her Olympic-standard embonpoint and inverted triangle face, she has a sexy head-girl haughtiness, and the many close-ups of her tensely appalled expression by the card table make it look as if she has witnessed Bond dissecting a frog on the green baize.
Who else but Loesser could have written "On a Slow Boat to China" (sung by Jason Danieley), whose jauntily lecherous come-on ("I'd love to get you on a slow boat to China") was an expression used by card sharks to describe their glee at playing with a loser?
On the other hand, individual expression with the card game means the influence of certain dominant members of the group is limited.
However, more effort should be put into elucidating the regulatory network among these genes via a strategy of system biology, integrating colorectal specific gene expression microarray, microfluidic card Q-PCR and proteomics study.
Regular Expression enables wild card matching of certain characters in the URL and is helpful if visitors land on the URL from a sub-domain.
eg. if your chromosomes are "chr1.fas", "chr2.fas", etc... Then the wild-card expression should be "chr*.fas".
There were some problems with the new wild-card expression for chromosome files so we have reverted to the old one and added an option in the.cfg file.
However, being part of a joint design process, in a welcome environment, with materials that ease the participants' expression (as drawings, cards, pictures), enriches the designer view for the context, promoting the design of solutions that make sense for the users.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com