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Our motivation to determine if intracellular signaling modifies BKα trafficking to increase or decrease surface expression calls for a new quantitative technique.
Likewise, gene expression calls for 24 down-regulated proteins were supported by 8 and 5 down-regulated genes in tumours and transgenic non-tumour livers, respectively.
The first component would reflect a 'gene expression memory' of the tumor of origin (similar expression calls for cells from the same origin, different calls for those from different origins).
Furthermore, gene expression calls for 45 up-regulated proteins were supported by 5 up- and 4 down-regulated genes identified in tumours and 4 up- and 6 down-regulated genes in transgenic non-tumour livers.
Level 3 data for DNA sequence mutation (somatic mutation calls for each participant), copy-number (putative copy-number calls, per sample), methylation (calculated beta values mapped to the genome, per sample), and mRNA (expression calls for genes, per sample) were used for analysis of different 'omics dimensions.
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The report, authored by David Kaye, UN special rapporteur on freedom of opinion and expression, called for greater protections for journalistic sources, as well as for journalists themselves — particularly those that publicly or privately expose malfeasance.
d) At least 6 probe-pairs should be used to derive an expression call for a transcript.
Level 3 released data contain quality controlled and processed data done by Broad Institute's TCGA workgroup with expression call for genes per samples.
On this type of approach, neither the concept of the many, nor the use of plural expressions, calls for modification to the doctrine that whatever is, is one.
For the cell lines assayed by both Tag-seq and qRT-PCR, measurements agreed remarkably well between the two technologies: the median Pearson correlation for expression profiles of individual genes was 0.91 and the differential expression calls were corroborated for all 82 genes.
Robust expression calls were detected for 12,463 of the 15,650 sequences targeted by the arrays, which were designed based on data from partial genome sequencing and expressed sequence tags from isolate 88069.
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