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Six out of the eight presumptive mesenteries express anthox6a, and the sharp expression boundary appears to coincide with one pair of mesenteries.
To identify cell migration by lineage labeling, we compared the location of cells expressing Foxb1 with the location of Foxb1-lineage cells: of necessity, any lineage-labeled cells found beyond the Foxb1 expression boundary had either migrated from the Foxb1-expressing domain or were derived from mitotic neuroepithelium that had expanded from the Foxb1-expressing domain.
Foxb1 is a transcription factor gene widely expressed in the neural tube, with a rostral expression boundary between caudal and rostral diencephalon (Kaestner et al., 1996; Wehr et al., 1997; Alvarez-Bolado et al., 1999, 2000a) which makes this gene useful for studying cell migration into the hypothalamic primordium.
Foxb1 is a transcription factor gene expressed in the neuroepithelium of the developing neural tube with a rostral expression boundary between caudal and rostral diencephalon, and therefore appropriate for marking migrations from caudal levels into the hypothalamus.
TBX3 is required downstream of HAND2 to refine the posterior Gli3 expression boundary.
Hox1 and Hox3 orthologs also share an anterior expression boundary in Nereis virens and the spider Cupiennius salei [65].
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The analysis reveals how the cross-regulatory network is able to establish sharp gene expression boundaries (among target genes that, if expressed in isolation, would have a graded or uniform expression profile).
UK laws outlaw hate speech and criminalise grossly offensive expression, but because there have been very few court cases over artistic expression, boundaries are often controlled by non-judicial considerations such as public opinion and taste, Index notes.
Using an engineered transcriptional repressor gradient, we demonstrate that overlapping repressor and activator binding sites provide more robust repression and sharper expression boundaries than non-overlapping sites.
Nereis and Platynereis lack posterior Hox gene expression boundaries, consistent an absence of morphological boundaries.
Furthermore, Nereis and Platynereis lack spatial colinearity of anterior expression boundaries, and only two larval segments have corresponding Hox gene expression boundaries (Figure 15, [11]).
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