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If one compares LdARL-1 and LdARL-3A [59], [59], LdARL-1/GDP- or /GTP-blocked expression does not impair the endogenous protein function, while LdARL-3A/GDP- or /GTP-blocked expression blocks the endogenous La/LdARL-3A function, resulting in a drastic flagellar shortening; on the other hand, LdARL-1/T34N (empty) is dominant-negative whereas LdARL-3A/T30N (empty) is inert.
These cells also display a greater number of asymmetric and prematurely terminated replication forks compared to controls, which is also observed in cyclin E expressing cells (Bartkova et al., 2006), demonstrating that oncogene expression blocks DNA replication fork progression (Di Micco et al., 2006).
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Tetherin is an interferon-induced protein whose expression blocks the release of HIV-1 and other enveloped viral particles.
EhCaBP1 is an essential protein, as down regulation of its expression blocks proliferation of the parasite [6].
In pdf01 mutants, shits1 expression blocks the effects on behavioral period but does not block the effect of shits1 on PER LNv rhythms.
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Inhibition of β1-integrin expression blocked local angiogenesis and reduced recovery from neurological deficit.
In addition, Immunofluorescence assay showed that NEDD8 expression blocked polη foci formation under oxidative stress (Fig. 6D and 6E).
In addition, NICD1 or Hes1 expression blocked erythroid differentiation (Kumano et al., 2001; Lam et al., 2000) and Notch1 inhibition using a loss-of function approach resulted in spontaneous erythroid maturation (Lam et al., 2000).
Knockdown of DDX41 expression blocked activation of the mitogen-activated protein kinase TBK1 (TANK-binding kinase 1) and the transcription factors NF-κB and IRF3 (interferon regulatory factor) by B-form DNA but not RNA.
Efforts to artificially increase Cdc45 levels through ectopic expression block cell growth.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com