Furthermore, cell labeling and extracellular matrix expression assays demonstrated that the transplanted hESC-MSCs not only contributed directly to tendon regeneration, but also exerted an environment-modifying effect on the implantation site in situ.
Gene expression assays demonstrated that integrin β3 mRNA was dramatically repressed by CDK11p58, and luciferase results confirmed that the integrin β3 promoter was inhibited by CDK11p58 through ERα repression.
Transient expression assays demonstrate that 2.2 kb of upstream sequence in either species is sufficient to drive GFP reporter expression that correctly mimics this pattern of Endo16 transcription.
The results of mRNA and protein expression assays demonstrate that fluoxetine can alter the inflammatory environment within the brain and stimulates cytokine production.
Its activity and involvement in CGA biosynthesis have been confirmed by heterologous expression assays, demonstrating that it can use either p-coumaroyl-CoA or caffeoyl-CoA as an acyl donor, and quinic acid as an acceptor.
In experimental invasion assay, demonstrating that ezrin over-expression is sufficient to confer metastatic capability.
A reverse transcriptase PCR assay demonstrated that the expression patterns of MrToll were distinctly modulated after Aeromonas caviae stimulation, with significant enhancement at 3 12 h post-challenge and a decline to basal levels at 24 h post-challenge.
The wound healing assay demonstrated that ectopic expression of miR-26b slowed down the migration of Huh 7 and Hep3B cells, while inhibiting endogenous miR-26b dramatically accelerated migration.
Luciferase reporter assay demonstrated that the co-expression of LMO3 and HEN2 attenuates HES1 (a negative regulator for Mash1 -dependent reduction of luciferase activity driven by the Mash1 -dependent
Additionally, flow cytometry assay demonstrated that the co-expression ratio of PPAR β/ δ and insulin was 14.4%, indicating that more than 93% INS+ cells expressed PPAR β/ δ at the terminal differentiation (Supplementary Figure S6).
