Sentence examples for expression ascertained from inspiring English sources

The ADRB2 mRNA expression, ascertained by quantitative RT-PCR [12], did not necessarily mirror β2AR protein expression for each haplotype (Figure 3A).

Cellular characterisation was completed on a particular EGFP-lamin A clone, known as SW480/lamA and a particular EGFP clone, termed SW480/cntl which were selected based on their moderate EGFP-reporter expression, ascertained by western blot.

In order to determine whether the loss of PP1γ expression was proteasome-mediated HPV-positive SiHa, CaSki and HeLa cells, and HPV-negative C33A and HaCaT cells were grown in the presence of the proteasome inhibitor, MG132 for 3 hours, after which the cells were harvested and the levels of PP1γ expression ascertained by western blotting.

For each gene model, the growth stage and the plant organ which showed maximal expression were ascertained and maximal expression profiles for growth stage and plant organs relative to the Arabidopsis gene models were constructed.

Expression analysis ascertained so far for variants I and II in normal tissues [ 25] shows prevalent expression of splice variant I.

β2AR expression was ascertained using a highly quantitative 125I-cyanopindolol (125I-CYP) radioligand binding assay.

The extinction of H4R protein expression was ascertained by FACS analysis after 24 h.

Before quantifying target gene expression, we ascertained that our LV samples had at least 10-fold upregulated levels of NPPA mRNA compared to controls (Figure S3, p = 0.008).

KIT expression was ascertained immunohistochemically and validated by immunoblotting.

PPARγ protein expression was ascertained by immunocytochemical analysis (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-1984) 1 : 50.

GFP and YB-1-GFP fusion protein expression was ascertained by detection with monoclonal GFP antibody.