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Fig. 3 Cytokine expressions in saliva samples according to (a) general diagnosis of OIIRR comprising of severe and moderate root resorption groups compared to CG and (b) specific diagnosis of moderate and severe OIIRR compared to CG. Cytokines that showed significant changes in their expression are identified by blue boxes.

Briefly, in pooled microarray datasets, a reference group of genes expressed above background with low variability of expression was identified by an F-test.

VEGF expression was identified by the brown cytoplasmic staining.

Genes with the most reproducible differential expression were identified by selecting genes in the initial and replication data sets with Log2FC≥1 and P≤0.05, and then assessing the combined statistical significance using the Fisher's combined P method [29].

Clones that affected reporter expression were identified by comparing intestinal GFP fluorescence with empty vector controls.

70 genes with differential expression were identified by both methods (Additional file 13C).

Receptor expression was identified by octreotide scintigraphy in 34 of 61 patients (56%).

Similarly, for Trichostrongylus vitrinus, 1,377 genes with sex-dependent expression were identified by microarray analysis [ 32].

Evidence of extensive antisense expression was identified by comparing the ESTs and MPSS transcriptional profiling data.

Pathways potentially regulated by v-Src-induced changes in gene expression were identified by Pathway Express software [ 40].

The clone that strongly silenced TXNIP expression was identified by real-time quantitative PCR (qPCR) and western blot analysis.