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The results are summarized in Table 2.> -wrap-foot> Individual gene expression (and validation).
We used the same sources of total RNA for both primary gene expression and validation experiments.
There is a need for biomarker expression and validation of these potential markers in large cohorts of patients.
The combination of immunoproteomic methods with protein expression and validation techniques provides an ideal basis for this highly demanding challenge.
All the transcripts with their expression and validation at different level such as genome hit and blast hit results against non-redundant database of NCBI.
This study was carried out on 482 preoperatively untreated rectal tumours, using a reproducible scoring method, a systematic approach to determining negative or positive protein marker expression and validation of prognostic effects by resampling of the data.
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To obtain a theoretical clarity, the shifting of FRF envelope phenomenon is incorporated into conventional FRF expressions and validation is performed with the FRF estimated using the Fourier Transform approach.
Global gene expression analyses and validation revealed that IL-15 regulated Tbx21 (T-bet) expression in thymic NKT cells.
Solexa sequencing and qRT-PCR were used for gene expression analysis and validation.
Three pooled tumours per each treatment condition were isolated on the fourteenth day of the treatment and snap-frozen for RNA isolation, gene expression analysis and validation study.
This promoter trap strategy can be exploited for expression analysis and validation of drug targets deduced from various non conclusive strategies such as bioinformatic analysis and microarray.
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