To show that the recombinant LOC100003999 and zgc:113054 genes are sufficient for encoding gadusol synthesis, they were cloned into a yeast expression vector and transferred into a Saccharomyces cerevisiae strain, in which the transaldolase gene TAL1 had been deleted.
The studies on antituberculosis vaccines involving plant expression systems and transferring the sequence of M. tuberculosis gene esat6 to plants are rather few [ 16– 216.
The IFN-CSP gene was cloned into the bacterial expression vector pET-21b and transferred into the expression strain E. coli BL21.
The upstream of OXA type class D carbapenemases in Acinetobacter is often associated with insertion sequence (IS), IS Aba1 and other IS may modulate the expression and transfer of OXA-type carbapenemase genes [ 5- 10].
This dataset is derived only from the following prediction approaches: neighborhood, transferred neighborhood, gene fusion, co-occurrence, transferred co-expression, text mining, and transferred text mining.
The "predicted functional associations dataset" consists of STRING PPIs that are generated only from the following prediction approaches: gene neighborhood, transferred neighborhood, gene fusion, and co-occurrence, transferred co-expression, text mining, and transferred text mining.
After 165 min, we induced cyclin expression and concurrently transferred cells to 34°C to inactivate the ndc80-1 allele.
RD29A promoter-inducible expression vector was constructed and transferred into tobacco plant.
A total amount of 75 μg (for measurement of Bax and Bcl-2 levels) or 40 μg of total protein (to measure TNF- α expression) were separated electrophoretically and transferred to PVDF membranes.
These chimeric constructs are easily excised and transferred into expression vectors that can be used to transfect a human CD4-expressing murine T cell hybridoma recipient.
