Furthermore, we have provided protocols and methods to monitor intracellular protein expression and trafficking as well as evaluation of the UPR, with essential controls.
N-cadherin is also thought to interact with the AMPA receptor subunit GluA2, thereby regulating the expression and trafficking of AMPARs (Saglietti et al., 2007; Nuriya and Huganir, 2006).
The VSPs and CWPs define distinct expression and trafficking patterns [26] [28], [32].
The present study confirmed the similar expression and trafficking of the human LAMP/Gag protein chimera.
Tetraspanins may regulate the expression and trafficking of their molecular partners.
The sequence of HCNCp is strikingly similar to the VSPs in many respects, however its expression and trafficking largely resemble that of the CWPs.
Therefore, ELuc is greatly advantageous for the achievement of high spatiotemporal definition BLI for the continuous visualizing gene expression and trafficking of proteins at the single-cell and subcellular levels.
Group 1 also contains the ERK/MAPK signaling pathway, a pathway known to work in tandem with the BDNF/Trk signaling pathway to regulate gene expression and trafficking during central nervous system (CNS) development [14] [15].
More recently, we have found that some antigens, including HIV-1 Gag, must be incorporated into the entire LAMP molecule in order to effect enhanced antigen expression and trafficking to the lysosomal compartment.
To test if the observed delayed assembly of PRE-dependent HIV-1 Gag could be attributed to a lower expression level compared with Rev-dependent HIV-1 Gag, the expression and trafficking of Gag-GFP in individual cells was monitored by live cell imaging after transfection with either Rev-dependent or PRE-dependent HIV-1 Gag-GFP.
Likewise, such studies may indicate factors influencing caveolin expression and trafficking from the Golgi, and elucidate their significance to the developmental cycle of these pathogens.
