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Heterologous expression and preparation of the recombinant protein was carried out according to [ 35].
Bacterial expression and preparation of GST-fusion proteins was performed according to standard procedures.
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The construction, design and preparation of pFLAG CMV4 mammalian expression vectors (Sigma-Aldrich) expressing M. tuberculosis Rv1818c CMV1818cc) and Rv3812 CMV38122) has been described previously [35].
NO was involved in design of the study, construction of cDNA library, in silico analysis, expression analysis with DNA microarray and preparation of the manuscript.
The expression of the corresponding proteins and preparation of microsomes were performed as described by Larbat et al. [ 11].
ML and MO performed cloning, expression, and antibody preparation of LOX-1, Western blot, and RT-PCR analysis.
JK performed RNA isolation, preparation of RNA for microarray hybridization of gene expression arrays, qRT-PCR experiments, analysis of array data and preparation of the manuscript.
The cost of lipases is determined by the catalytic activity, expression level, and preparation method.
Expression plasmids: The preparation of plasmid constructs expressing PPARα, γ and δ has been described previously.
The expression and inclusion body preparation of Xenopus laevis histones H2A, H2B, H3 and H4 with amino acid residue substitutions H3 C110andnd H4 T71C, were as previously reported [ 37] with modifications [ 18].
This is due to challenges in the expression, purification, and sample preparation of eukaryotic membrane proteins.
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