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In summary, we uncover female-specific, maternal diet-mediated in utero misprogramming of offspring metabolic balance with a striking sexual dimorphism in glucose- and maternal diet-sensitive PVH gene expression adjustment.
It also indicates that RNAseq, because of its high sensitivity, disregards the background epigenetic expression adjustment machinery associated with RNAPII phosphorylation.
We also show that our algorithm can be used in conjunction with expression adjustment techniques to produce a more biologically sound gene-state call.
Strikingly, female mice show no PVH gene expression adjustment in response to hypoglycemia.
In summary, we uncover female-specific, maternal diet-mediated in utero misprogramming of offspring glucose homeostasis and a striking sexual dimorphism in glucose- and maternal diet-sensitive paraventricular hypothalamus gene expression adjustment.
We cannot rule out that other sets of PVH genes do alter with glycemic state in female mice, but nevertheless, such stark sexual dimorphism in either gene expression adjustment or gene set usage to glycemic state has not, to our knowledge, been reported previously.
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