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Bar graphs display the fold change in each RNA (y axis) normalized to the expression in cells expressing vector control.
(A ) qRT-PCR analysis monitoring INK4-ARF expression in WI-38 cells expressing vector or KRAS(G12V).
NB cells were analyzed after transfection with PKCδ expressing vector in order to test the kinase expression pattern.
Cells expressing the mutants did not grow to the same OD600 as cells expressing vector alone, an anticipated effect of protein over-expression.
(C ) qRT-PCR analysis monitoring expression of USP28 and PRKD1 in WI-38 cells expressing vector or KRAS(G12V).
expressing vector.
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A single point mutation was engineered within Annexin A2-expressing vector, with resulting vectors called pEXS-Annexin A2-K302A and pEXS-CG-Annexin A2-K302A.
This was also confirmed by our double fiber-expressing vector Ad5/R7-ZZwt/E1 WTFib.
The vector pDsRed-Monimer-N1 and human erbB3-expressing vector pDsRed-erbB3 were described previously.
Hence the Ad5AMUCH_RSV-NIS CRAd could be used as a non-replicating NIS-expressing vector.
In this study, we injected the IFN- α-expressing vector locally to the tumour mass.
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