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Transfectants were selected either for their high CEA secretion (clone CO115-2C2 secreting up to 13 μg CEA per 10 cells within 72 h) or for their high CEA membrane expression (clone CO115-5F12 expressing up to 1 × 10 CEA molecules per cell).
Oottamasathien and colleagues reported that ESCs combined with fetal embryonic mesenchyme were able to form bladder-like structures expressing UP following 42 d of incubation within a kidney capsule model [64].
Phage display is a powerful combinatorial technique for peptide discovery that uses methods of recombinant DNA technology to generate a complex library of peptides, often expressing up to 107 109 unique sequences, that can bind to cell surface targets [16], [17].
Analyses of the corresponding xenografts revealed a significant reduction of cell numbers expressing the investigated surface markers and showed single cell fractions expressing up to three markers simultaneously.
The FPKM gene expression values ranged from 0 to 24.21 FPKM for the first to third quartiles, with several outliers, such as beta-2-microglobulin (B2M), expressing up to 17,330.00 FPKM (Supplementary Fig. 1).
Cotransfection of the primary human amniocytes with a plasmid expressing adenoviral E1 functions plus a second plasmid containing a gene of interest resulted in permanent cell lines expressing up to 30 pg/cell/day of a fully glycosylated and sialylated protein.
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Transgenic plants expressing up-regulatory Apl1 genes showed greater AGPase activity and turnover of transitory starch than wild-type plants, the latter resulting in elevated carbon and energy to support heterotrophic growth during the night.
A gene was counted as differentially expressed (up- or down-regulated) by expression level of at least one unique probe.
We then detected the pFAK and tFAK expression in the GC transfected cells that expressed up- or downregulated MGr1-Ag/37LRP adhesionhesion to ECM components and control.
We calculated the correlation coefficients between the expression profiles of these TFs and the differentially expressed (up- and down-regulated) genes in saliva in all samples.
moDCs were transduced to express up to three KSHV ORFs (grouped according to their expression profile) and then cultured with autologous CFSE-stained T cells.
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