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Each immunogen was produced in Nicotiana benthamiana plants using twin magnICON vectors expressing the light and heavy chains of the idiotypic Ab.
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Neurons were asymmetrically infected with lentiviruses expressing the light-activated cationic channel channelrhodopsin-2.
Previously, we demonstrated that depolarizing astrocytes expressing the light-responsive channelrhodopsin-2 receptor (ChR2) elicited CSD in brain slices and in anesthetized mice.
To test this, we took an optogenetic approach by expressing the light-driven outward proton pumps archaerhodopsins (Arch) (Chow et al. 2012) specifically in AIM interneurons using the zig-3 promoter.
By expressing the light-responsive ChR in neurons using cell-type selective promoters or other methods, specific neurons can be depolarized by light in a temporally precise manner with millisecond resolution even if their membrane biophysical properties are less favorable for electrical stimulation.
The method is based on expressing the light-activated channel channelrhodopsin-2 (ChR2) to restricted neuronal subpopulations.
But those stolid words do not express the light and lacy prettiness that made theValentino show the essence of summer.
A Tricistronic vector utilizing internal ribosome entry site (IRES) elements to express the light chain (LC), heavy chain (HC), and a neomycin phosphotransferase (NPT) selection marker from one transcript is designed for generation of mAb expressing CHO cell lines.
In this study signal sequence of amicyanin, which is encoded by the mauC gene of Paracoccus denitrificans, was used to express the light chain variable domain of the human κIO8/O18 germline antibody in the periplasm of Escherichia coli.
All of these organs express the light AChE species which is abundant in human plasma.
Additionally, we expressed the light chain individually, evaluated the effect of using different non-coding regions (NCRs) on the expression level and investigated the influence of temperature on the influenza replicon activity.
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