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This study has confirmed the feasibility of the microbial synthesis of the bulk chemical 2-HIB from hydrogen and carbon dioxide by exploiting the chemo-litho-autotrophic metabolism of C. necator H16 PHB−4, additionally expressing the foreign 2-HIB-coenzyme A mutase.
Young and aged C57BL/6J mice were treated with AAV8 vectors expressing the foreign antigen luciferase.
Finally, we tested whether dnaJ-H induction was specific to elevated dSir2 by expressing the foreign GFP mRNA using the strong, ubiquitous, constitutive daughterless-Gal4 driver and measuring dnaJ-H levels (Fig. S3).
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A high ratio of amplicon/helper virus HF10 (A/H) (>1) was required to express the foreign gene efficiently.
Furthermore, in order to express the foreign gene conditionally, an HSV-1 ICpromoterter was introduced in place of the human cytomegalovirus MIE promoter, this driving expression of the transgene when replication of HF10 progressed.
Cytotoxic T cells, in turn, can attack and kill other cells that express the foreign antigen in association with class I MHC molecules, which as explained above are present on almost all cells.
Genetically engineered animals and, the Cree/lox system, enables the production of conditional knockout animals that only express the foreign gene in selected tissues, and the production of inducible knockout animals in which expression of the mutant gene can be turned on at the developmental stage determined by the investigator.
Our host vector system strongly expresses the foreign gene.
In situ hybridization histochemistry revealed that microprojectiles penetrated through multiple cell layers without evidence of tissue injury and that 10-20% of the cells in the bombarded area expressed the foreign gene.
However, the growth rates of the transformants carrying pHFGE-1 and the widely used pG-1 were the same and both transformants strongly expressed the foreign gene, even in medium containing glucose, which was expected to repress expression of the foreign gene.
All mutants other than cbbLS::kanr contained near wild-type levels of the respective CbbL protein (Figure 4), indicating that the growth defect of the cbbL::Tc NC cbbL and cbbLS::Tc NC cbbLS mutants could not be explained by a failure to express the foreign CbbL.
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