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Similar expression patterns for anti-mouse GAT4 were found in the GAD67-GFP expressing taste cells (Figure 10).
We also used a transgenic mouse line that expresses GFP in GAD67 expressing taste cells which identify a subset of Type III taste cells [24], [25].
Some immunoreactivity overlapped with the IP3R3-GFP expressing taste cells (Figure 6A D, see arrowheads) while other IP3R3 expressing cells (see arrow) were not immunoreactive for GABAB1.
Since gustducin is found in a subset of PLCβ2/IP3R3 expressing taste cells [78], we predict that the GABAB2 labeling is likely present in the Type II taste cells that do not express gustducin.
We also used transgenic mice that express green fluorescent protein (GFP) in either the Type II taste cells, which can respond to bitter, sweet or umami taste stimuli, or in the Type III GAD67 expressing taste cells.
However, since its expression in other cell types has not been rigorously characterized and electron microscopy studies have demonstrated that almost all IP3R3 expressing taste cells are Type II cells [57], for this study we are presuming that the presence of IP3R3 identifies the taste cell as a Type II cell.
Similar(47)
Although the SCCs express taste receptor proteins, the sensations elicited by chemical stimulation of the SCCs are not tastes, but rather one of pain or irritation.
Several groups have demonstrated the presence of additional glucose-sensing molecules in both lingual and enteroendocrine cells that express taste receptors, including components of ATP-sensitive K+ channels (SUR1 and Kir 6.1), GLUTs, sodium-glucose linked transporter 1, and glucokinase (23, 24).
Interestingly, the chemokine CXCL14 is the highest expressed taste-bud associated gene in our database.
Yet not all cells that express taste-related transduction molecules should be considered to be SCCs in the original sense of the phrase.
They find a diversity in the behavioral dynamics evoked by different subsets of gustatory neurons expressing different taste receptors, suggesting that larvae can discriminate between tastes of the same quality because these evoke kinetically different behavioral responses.
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