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Genetic OR gates can be achieved by designing tandem promoter genetic circuits or by expressing target genes in two discrete expression cassettes.
For expressing target protein(s) in C. glutamicum, AGAAGGAG was used as normal RBS sequence in our previous studies.
The schema mapping alone is not adequate for expressing target semantics, and hence, the canonical instance is in general not optimal.
Recently, we demonstrated that E5-tagged epidermal growth factor (EGF), when bound to a K4 expressing adenovirus, promotes retargeting of the adenovirus to EGFR expressing target cells.
In addition, several sequences such as GAAAGGAGA, GAAAGGCGA and GAAAGGA were used as strong RBS for expressing target genes in C. glutamicum (Kang et al. 2014; Zhang et al. 2015b).
In contrast to the dominant T helper (Th) 1 responses which have been observed using rMVA expressing target Ags, mice immunized by the intramuscular (i.m).
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We postulate that immune effectors inappropriately activated in autoimmune individuals are capable of protecting against tumor expressing targeted autoantigens.
A selection of patients expressing targets for imatinib treatment is of great relevance.
This treatment abrogated the enhanced adhesion to E-cadherin expressing targets, confirming that the increased adhesion to E-cadherin expressing targets was due to the expression of functional CD103.
Cost-effective production of soluble recombinant protein in a bacterial system remains problematic with respect to expression levels and quality of the expressed target protein.
In the first system, small granules of polyhydroxybutyrate (PHB) are produced in the expression host, which then act as an affinity carrier for the subsequently expressed target protein.
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