The contact angle which is the angle on the contact interface between the liquid and solid is a popular index for expressing surface wettability of general materials such as metals, glasses, and resins [22].
It follows that stimulated T cells activate different monocyte functions by expressing surface molecules.
Briefly, cell cultures expressing surface c-MET receptors in the presence of either exogenous or endogenous HGF are treated with a membrane impermeable sulfo-EGS cross-linker.
The effects of drugs that may affect deregulated signal transduction pathways in leukemia can be readily tested in cell populations expressing surface epitopes examined in this study.
The resulting cells expressing surface γδ TCR were evaluated by flow cytometry with fluorescein isothiocyanate (FITC -conjugated anti-human γ9 Biolegend and phycoerythrin (PE)-conjugated anti-human δ2 (Biolegend) monoclonal antibody.
Eighteen hours post-treatment the expression of CD4 levels at the surface of Mφ was barely detectable (Fig. 2A), and the percentage of Mφ expressing surface CD4 was significantly reduced by 4-fold (Fig. 2B).
