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Cells expressing reaction for macrophage marker (CD68) showed on the other hand often tachykinin-like immunoreactivity.
Founded upon expressing reaction fluorescence in relation to amplicon DNA mass, a seminal element of this study was to implement optical calibration using lambda gDNA as a universal quantitative standard.
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In particular, where tables and figures identify which pathways and which genes are enriched or differentially expressed, reaction norms visualize how this enrichment or differentially expression differs under different forms of stress or between different genotypes.
The MC index, on the other hand, measures the amount of coordinated (connected) expressed reaction structure, which can only be observed after contextualization of the expression data.
In particular, AcP is transformed to Ac through the highly expressed reaction acetate kinase (ACKr).
The elementary flux mode (EFM) framework is an approach to express reaction pathways contained in metabolic networks [ 1].
This difference was due to either the unexpressed reaction being added back in for growth rate requirements or that the expressed reaction was a false-positive in terms of the entire network.
If a non-core reaction r j is connected to a highly expressed reaction r i that has few other connections, this provides strong support for its inclusion in the context-specific model.
Conversely, enzyme kinetics express reaction rates as complex functions of metabolite concentrations and enzymatic activities, which vary over time as a result of transcriptional and metabolic regulation (Smallbone et al., 2007).
Ignoring the reconstruction context, the gene expression data predicted 27 and 40 exclusively expressed reactions in the normal and diabetic obese patients, respectively.
Thus, the context-specific models predicted 95 differentially expressed reactions due to stoichiometric and flux constraints that the expression data could not solely predict.
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