In the following protocol a procedure is exemplarily described how to create transgenic plant cells expressing nucleus-targeted aequorin and to monitor any changes of Ca2+ concentrations in this particular compartment.
They estimated that in the highest expressing nuclei, Bicoid is present at a concentration of 55±3 nM, or 33±1.8 molecules/μm.
(H ) Intensity profile of LAP2α (red) near telomeres (TRF1, green) in TERT+ human fibroblasts, control (left part) or progerin expressing nuclei (right part) along a ∼400 nm axis, relative to the minimum and maximum LAP2α and TRF1 signal per nucleus.
Then one can show that not only are the binomial and Poisson estimators of ν biased high, but also the bias increases for higher expressing nuclei (proof straightforward; omitted).
For all drugs used in this study, the dose-response curves for Ki67 expressing nuclei of proliferating cells matched the ones for the total nuclei count, suggesting that the drugs tested were cytotoxic rather than anti-proliferative.
In cultures from all individuals tested, derived from 13 different biopsies, the number of DUX4-FL expressing nuclei remained stable upon repeated subculturing, indicating that the mechanisms regulating DUX4-FL expression are similarly stable in myocyte cell culture.
P63 expressed nuclei contain important information related to histologic grade.
Textural features were generated using the gray-scale version of the P63 expressed nuclei.
Figures 1(a) and 1(b) present the original and segmented (P63 expressed) nuclei images.
A number of textural and morphologic features were computed from the P63 expressed nuclei.
Nuclei in the first image attained brownish colour (expressed nuclei) and in the second image bluish colour (nonexpressed nuclei).
