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Fluorescent excitation and emission spectra of EGFP expressing living cells were arithmetically folded with excitation and emission filter transmission data allowing the calculation of fluorescence yields for different filter combinations.
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Carers expressed living with feelings of guilt in all trajectories.
Now, attempts to express lived facticity occurred not infrequently in philosophy over the course of the last century.
Surprisingly, SOCE was unaffected in E329Q-NSF expressing live and adherent cells even 20 hr after inducing the expression of the mutant and unlike α-SNAP, WT-NSF failed to co-localize with Stim1 clusters in ER PM junctions.
We first visualized the embryonic BBB using the endogenously expressed live-imaging markers Nrg-GFP and Lac-GFP (Morin et al., 2001).
Unlike small-molecule fluorescence biosensors, fluorescent proteins are genetically encodable, and thus can be expressed inside living cells.
The exploitation of RNA molecule as functional molecular devices is expanded from logical gate constructs to the fabrication of functional synthetic supramoleculer RNAs that have been expressed in living cells.
The fusion construct (TaSnRK2.8 GFP) driven by the CaMV 35S promoter was transiently expressed in living onion epidermal cells.
Thus, mCerulean3 performs well as a fusion protein and displays very little fluorescence photoswitching when expressed in living cells.
Moreover, iAbs are also powerful molecular tools for probing the functions and interactions of their targets when expressed in living cells.
To compare the ability of these two red cameleons expressed in living cells, we transfected HeLa cells with the same amount of cDNAs encoding either SapRC2.12 or SapRC2.
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CEO of Professional Science Editing for Scientists @ prosciediting.com