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These data show that retrovirus vectors expressing from the EF1α ubiquitous or MeP restricted promoters can convert virtually all +/− NSC into MeCP2+ cells.
To test whether increased α3 connexin proteins expressing from knock-in α8(Kiα3/Kiα3) alleles can prevent a severe nuclear cataract caused by the γB-crystallin S11R mutant proteins expressing from γB S11R/S11R) alleles, we generated compound mutant γB S11R/S11R) α8(Kiα3/Kiα3) mice.
To determine the in vivo function of PiT1, we generated an allelic series of PiT1 mutations in mice by combination of wild-type, hypomorphic and null PiT1 alleles expressing from 100% to 0% of PiT1.
The methodology allows for an analysis of the representation of autoreactive B cells in different B cell subsets in a far larger number of patients than can be examining in methodologies that rely on cloning antibodies and expressing from individual B cells.
We can solve this dilemma by inserting the cloned genes into the chromosome rather than expressing from a plasmid.
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Although most ESAGs are expressed from telomeric expression sites [ 41- 43], there is evidence that some ESAGs can also be expressed from chromosome internal regions of the genome[ 47].
This contrasts to previous studies in which the constitutive hacA was expressed from a highly-expressed promoter [ 43] or expressed from the pyrG locus [ 30].
MiR-19 repression of the 19RE reporter expression was defined as the luciferase activity expressed from the REm reporter divided by that expressed from the 19RE reporter.
We tested expression of Turbo, tdTomato, mCherry, mKat and mPlum expressed from Phsp60 or Psmyc.
Notably, BLIMP1 (expressed from 6.5-dpc mouse PGCs) was concomitantly upregulated, whereas STELLA expression (expressed from 7.5-dpc mouse PGCs) was not significantly altered.
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