Metagene analysis was conducted using gene expression microarray data from cell lines constitutively expressing empty vector, WT PR, or K388R PR, and treated with either vehicle or R5020.
To gain insight into possible mechanisms underlying the observed miR-9-dependent invasive behavior of mast cells, we compared the transcriptional profiles of murine BMMCs overexpressing miR-9 to those expressing empty vector and found marked changes in gene expression.
Furthermore, the presence of exogenous Bcl-3 sustained MMP-1 expression in the presence of CDDO in comparison with cells expressing empty vector.
Whole-genome expression data were ranked according to fold upregulation comparing A172 glioblastoma cells expressing empty vector (EV) with and without BV6/TMZ treatment.
Authenticated immortalized human mammary epithelial (HMLE) cells expressing empty vector (pWZL), Snail, Snail shControl, or Snail shFOXC2 cells were cultured in MEGM media as previously described (Werden et al., 2016).
M2 cells stably expressing empty vector (pcDNA3), wild type FLNa, or FLNaΔ3 (repeat 3 deleted) were produced (Fig. 3A insert).
In agreement with above, depletion of USP19 in DU145 cells expressing empty vector led to an induction of p27Kip1.
FASN and Myr-AKT overexpressing cells were monitored for p63 knockdown- induced cell death against cells expressing empty vector.
On the contrary, BaF3 cells expressing FLT3-ITD is not sensitive to TAE684, neither did BaF3 cells expressing empty vector (Neo-Myc).
Acute insulin stimulation of Akt phosphorylation was restored in the three TSC2-reconstituted lines but not in cells expressing empty vector, which remained insulin resistant.
Peak calcium release in response to UTP did not differ between control cells expressing empty vector, cells expressing wild-type APP, and cells expressing APP mutants (Figure 2B).
